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To determine if a discrete portion of this region binds ferritin, we made further truncation constructs and demonstrated binding to amino acids 475 522.
Thermal denaturation and CD titration studies demonstrated binding to the cognate sequence (5′-AAGCTA-3′).
TS1 exhibited a distinct cytoplasmic pattern of staining in viable tumour cells, but also demonstrated binding to necrotic regions and pyknotic/apoptotic tumour cells (Figure 1).
In vitro fluorescence microscopy evaluation of cKNGRE-OG demonstrated binding and active uptake by CD13+ cancer cells and minimal binding to CD13− cancer cells.
The Foxp3 gene contains several conserved potential STAT5 binding sites, some of which demonstrated binding of STAT5 in Treg but not Tconv (Burchill et al., 2007; Yao et al., 2007).
Second, tau protein is demonstrated binding to the minor groove of dsDNA without nucleotide sequence specificity (Figures 4 6).
Further, we have also demonstrated binding of a biotinylated-BH4-BCL2 peptode to cells in vitro (Fig. S4).
HLH-1 ChIP-qPCR demonstrated binding slightly above background for only one of these 31 negative control amplicons (Figure 4C).
Because our studies previously demonstrated binding activity of a Myo Va construct containing the neck region (654-1402) [16], we constructed three additional neck mutants (Fig. 1A).
Pull-down assay demonstrated binding of nucleolin and Stat1 in M-CSFR expressing THP-1 cells stimulated with CSF-1 (Fig. 3B).
Ligand affinity blot demonstrated binding of CFH to G1/pCRASP-3 and G1/pCRASP-5 but not G1 and G1/pKFSS1 (Fig. 3E and F, respectively).
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