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Respondents demonstrated attachment to the dam-in-place landscape, even those initially disadvantaged by its construction, and a preference for keeping the headpond intact.
Moreover, it also demonstrated attachment to human respiratory tissues, although this attachment was not as extensive as that exhibited by the control 2009 pandemic CA/07(H1N1) virus.
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To demonstrate attachment to other solid supports a beaded agarose affinity support (Affi-Gel 102 resin, Bio-Rad) was modified with oligoglycine by incubating the amino-resin with diglycine (0.5 M) and EDC (2.5 mM) for three hours at 50°C.
These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used.
Attachment to surfaces located at a central leaf region demonstrated intermediate attachment level (7.0 log CFU g−1).
In addition, applying the ECIS technology to monitor IEC adhesion on different substrates in real time, we demonstrated that attachment and spreading of Caco2-BBE cells on recombinant CD98 were enhanced when CD98 was phosphorylated.
Seeding studies demonstrated EC attachment at 30 min, with progressive improvement at 1 and 2 h (47, 72, and 89% attachment, respectively).
We have previously demonstrated that attachment of the fluorescent proteins at these sites does not interfere with the activity of SERCA21,63.
Scanning electron microscopy (SEM) demonstrated cell attachment (average of 3.0×108 cells per bead) to the irregular surface.
Human endothelial cells demonstrated strong attachment to the composite PCL PU scaffold, and proliferated to form a monolayer with strong PECAM-1 expression and cobblestone morphology.
For seeding densities of 5×104 and 1×105 cells cm−2, PET and plastic demonstrated >95% attachment of seeded numbers after 3 h.
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