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In order to demonstrate whether the effect of galanin on 5-HT neurotransmission is mediated through galanin receptors, the peptidergic galanin receptor antagonist M35 or aCSF were infused 120 min before the administration of galanin.
The results could not demonstrate whether the effect of age of onset as measured in our study indicates a trait or state characteristic.
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The main work here is to demonstrate whether the effects of transcriptional interactome on gene co-expression exist on genomic level.
To demonstrate whether the effects of derlin-1 on cell survival are specific to ER stress or were a general mechanism of stress resistance, the same cells were treated with 1 μM staurosporine for 24 hours.
In order to demonstrate whether the effects of oestrogen and tamoxifen were direct, ER positive patient derived cells (primary 2) and MCF7 cells were FACS sorted to assess the oestrogen receptor status of the CSC enriched ESA+CD44+CD24low sub-population (Additional file 3, Figure S2 and Additional file 4, Figure S3 show gating procedure and example. For more details see [ 18]).
Therefore, we want to demonstrate whether the 'migration effect' on the ability to acculturate exists.
Taking this in consideration, we used VPA to demonstrate whether the adverse effects observed in vivo may be also recapitulated to some extent the in vitro conditions using the hESC differentiation model.
To demonstrate whether the induced effects of rhPDGF-BB on proliferation and osteoblast differentiation of diabetic BMSCs is dependent on ERK pathway, we used PD98059, a specific inhibitor of MEK.
However, more studies are needed to demonstrate whether these effects are harmful or/and affect the neural functions.
Further studies are required to demonstrate whether the favorable hemodynamic effects of levosimendan, as also demonstrated in the present study, are clearly potentiated by its anti-inflammatory action.
Then, in order to conclusively demonstrate whether the observed immune regulatory effect was indeed specific for NPCs, we challenged the capacity of other somatic stem cells, such as bone marrow derived MSCs and vessel-associated mesoangioblasts (MSAs), of interfering with the expression of co-stimulatory molecules, when co-cultured with DCs maturating in vitro with LPS.
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