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All of these comparisons demonstrate that our procedure is more effective at identifying miRNA-gene comodules.
We first demonstrate that our procedure overcomes the well-known problem of antibody penetration in compact structures by performing immunostaining of EpCAM, a membrane protein expressed by all cells within our spheroids.
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"I think this demonstrated that our procedures are good and our process is good.
Results demonstrate that our correction procedure clearly improves the BFS-method as it is able to reveal a hierarchical structure without inconsistencies.
We demonstrate that our ConReg-R procedure can significantly improve the estimation of FDR on simulated data, and also the environmental stress response time course microarray datasets in yeast and a human RNA-seq dataset.
Overall, this resampling analysis demonstrates that our sampling procedure quickly discovers the larger communities in the network if they are not completely isolated.
This is consistent with previous findings [ 4, 53], which demonstrates that our experimental procedure has succeeded to identify endogenous small RNA in human cells.
For instance, our group was able to perform the cross-modelling of two different murine MHC-I alleles, H2-Db and H2-Kb, demonstrating that our modelling procedure with Modeller software was able to reproduce all aspects of the MHC-I cleft, despite the known differences in the template (11).
Overall, this demonstrated that our RNA-seq procedure reliably identified region-specific target genes, apart from the anterior Hh set.
The simulated values of diffusion coefficients and kinetic parameters were consistent with experimental data from literatures, demonstrating that our Monte Carlo simulation procedure is useful for kinetic analysis of cellular signal transduction.
To demonstrate that this procedure yielded multi-potent MSCs in our hands, we performed limit dilution and placed single cells into separate wells of a 96-well plate and allowed them to proliferate.
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