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We report a new, high‐affinity dopamine sensor as well as the first MRI reporter for serotonin, with which we demonstrate quantification of neurotransmitter release in vitro.
We further demonstrate quantification of botulinum toxin in both exogeneous (human blood and serum spiked with toxins) and endogeneous (serum from mice intoxicated via oral, intranasal, and intravenous routes) samples.
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In contrast, the system described herein demonstrates quantification of MDR1 substrate and modulator activity in parallel and at single cell resolution.
A few reports of one-step digital RT-PCR have demonstrated quantification results that agree well with those of other quantitative or semiquantitative methods using RNA standards or standard virus quantification methods to indirectly quantify RNA for comparison with system performance.
We demonstrate accurate quantification of BR accumulation by ratiometric analysis of BR (Amax 568) and a membrane-bound cytochrome (Amax 410).
Hepcidin, a peptide marker of clinical disorders linked to iron metabolism, was used as model to demonstrate peptide quantification potentialities of LC-chip coupled to a nanoelectrospray source ion trap mass spectrometer in an aqueous sample.
The portable, commercially available ArrayCAM™ was used to demonstrate the quantification of three different fluorophores labeled for IgG, IgA, and IgM due to the discrete emission quantum dot spectra.
However, these techniques demonstrate cDNA quantification and may not reflect the actual quantity of RNA present in the original sample.
However, one needs to demonstrate that quantification is not affected by CT truncation even when truncation corrections are applied.
A representative HPLC-MS/MS chromatogram is shown to demonstrate the quantification of DNA adducts detected in vivo.
Thus, these analyses demonstrate that quantification of DAPI fluorescence intensities is a valid method for estimation of relative DNA mass.
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