Furthermore, these data demonstrate one more function of RA T cells; namely, their ability to induce monocyte/macrophage chemokine secretion by cellular contact.
The karyotype of CNS-1 cells demonstrates one or more trisomies often of chromosomes 11 and 13 (Kruse et al., 1994).
Figure 2 demonstrates one example.
19 cells of H9 MP cells demonstrated an apparently normal female karyotype while one cell demonstrated a non-clonal chromosome aberration (45, XX, −20), which is most likely an artifact of culture.
STI-1, labelled with Cy3 (red) or FITC (green) fluorochromes, and cell-type-specific markers, Neu-N, MAP-2, Tuj-1, vWF, GFAP and HIF-1α, labelled with Cy3 (red) or FITC (green) fluorochromes were double immunostained in order to demonstrate their co-localization in one cell under laser-scanning confocal microscopy.
To create compound profiles, compounds that demonstrated activity in at least one cell type were clustered by their IC50 values across the 13 cell types.
Subsequently, many researchers started to focus on miRNAs in EVs, and accumulating evidence has demonstrated that EVs transfer miRNAs from one cell to another and that their components have an effect on cancer progression [18, 19].
A total of 56 genes (of 11 000 transcripts analysed), each with a 5′ CpG island, that demonstrated >5-fold increased expression in one cell line or >2-fold in multiple cell lines were identified as candidate epigenetically inactivated RCC TSGs.
To demonstrate that this outcome was not restricted to one cell line, we substituted MCF-7 cells for pLUM cells.
To convincingly show that a given subset has regulatory rather than effector function, one should demonstrate suppressor cell activity in vivo in an animal model as described by Hippen and colleagues [ 11] with human cord blood CD4+CD25+ cells.
