First, there's the house itself; not only has he inherited the physical property, but also his grandfather's "field-of-care" – a magical demarcation zone running around the surrounding fields and paths.
In the absence of a demarcation zone with peacekeepers, it is unlikely that anyone can ensure full compliance on this point, but the most important thing is that both sides stop shelling each other.
Lung samples were taken from three different sites: necrotic area, demarcation zone, and visually unaffected area.
RT-qPCR, on the other hand provides additional, complementing results for the demarcation zone as well as a control sample group.
No significant difference of the miR-15a expression has been detected between the infected samples (visually unaffected, demarcation zone, necrotic), which is also reflected in the RNAseq results.
In addition to RNA isolated from visually unaffected and necrotic tissues, RNA from the demarcation zone (between infected and non-infected tissue), and from nose and trachea originating from the same infection study were included in the RT-qPCR studies.
The expression levels of all assayed microRNAs but one and of the snoRNA in the demarcation zone sample are intermediate in relation to the visually unaffected and necrotic areas.
On XY′ telomeres, the distal Y′ area is organized into chromatin that resembles that of expressed areas with the X element, again acting as a demarcation zone (Fourel et al. 1999; Pryde and Louis 1999; Takahashi et al. 2011).
Taking the nature of the demarcation zone sample (tissue sampled at the border of necrotic and visually unaffected tissue, see Figure 5) we would expect the intermediate microRNA expression rates.
Total RNA was extracted from each necrotic lung, visually unaffected lung, demarcation zone lung, trachea, nose, F50, F100 and control porcine sample, using Tri Reagent® (Molecular Research Center, Inc., USA) following the manufacturers recommendations.
