Pathologists have a good ability to estimate ratios of clearly demarcated areas, but gradients in staining intensities hinder reproducible visual demarcation of positive tumor areas.
Most histological assessments involve either counting of positive stained cells or demarcation of areas of damaged tissue.
Sections of the heart (myocardium) served as positive controls and β-tubulin for demarcation of areas containing immature neurons.
The demarcation between areas staining positively and negatively by IHC was sometimes abrupt, as seen for FAP staining in Figure 2A, left.
"There was no cross contribution between tissue types or germ layers". In other words, there were clear demarcations between areas of color that corresponded to structures such as the epidermis, tendon, nail, vessels, nerves and bone.
The demarcation of study area was based on 2 km buffer of Ranchi Municipal Corporation boundary, which covers majority of continuous built-up land of RUA as observed in satellite image of the year 2010.
Although quantitative methods of characterizing cortical areas [36] [38], [40] enable observer-independent area demarcation, the area identification is still subtle and requires exact spatial information of the section of interest.
This task is, in fact, quite difficult, especially for the rodent cortex, where there are no clear-cut borders for area demarcation.
We emphasize that the cytoarchitectonic areas determined by Nissl-GLI are well consistent with the expression patterns of the layer-specific genes, validating the area demarcation by our method.
Lung samples were taken from three different sites: necrotic area, demarcation zone, and visually unaffected area.
