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Furthermore, plasma, an abundant source of glucose and glutamine in cancer cells, was represented by two demand reactions in the reconstruction, see Figure 1 and Table S1.
Hence, sink and demand reactions were included to define proper metabolic boundaries to mimic the physiological conditions prevailing around cancer cells, see Table S1.
In turn, through demand reactions (which are unbalanced reactions that permit the accumulation of a compound otherwise not allowed in steady-state models because of mass-balancing requirements), we were able to include a source of ACCOA, ADP and oxygen.
For gaps that have demand reactions in the model, the demand reactions were constrained to zero before running SMILEY.
Instead, they were modeled via the addition of demand reactions to allow their accumulation during simulation.
In approach B (modelB), we included only genes and their reactions homologues to Recon 1 genes as well as transporter and demand reactions.
Similar(46)
In case a partial or full pass through occurs, it is reasonable to assume that a demand reaction will follow, depending on the extent of the price increase and the price elasticity of demand.
Kuo et al. ([2003]) used the same period as Heeb et al. ([2003]) with the price reduction due to the WTO agreement to estimate demand reaction to the price decrease.
Everything becomes heightened, we demand response, we demand reaction to these things that are terrible and happening right in front of us on the web.
Thus, for example, studies on C13 NMR spectroscopy have demonstrated that glutaminolysis constitutes an active metabolic pathway in human glioblastoma cell lines [8], and consequently, a demand reaction of α-ketoglutarate representing an intermediary compound along the conversion of glutamine to lactate was included in the reconstruction.
Another common situation is where cofactor cycles such as ATP ADP or NADH NAD + link supply and demand reaction blocks.
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