ERBB2 transcription was quantified using an Assay-on-Demand mixture (Applied Biosystems, Foster City, CA, USA).
My perfect brownies demand a mixture of cocoa powder and chocolate, for ultimate richness of flavour without heaviness, and intensive beating, to give them an irresistibly crisp crust.
While many middle-skill jobs are susceptible to automation, others demand a mixture of tasks that take advantage of human flexibility.
Still, running a theatre - any theatre - always demands a mixture of expediency, experience and luck, so Nunn's fiercest critics might want to think twice before hysterically demanding that he be ousted.
Clearly, justice demands some mixture of the Swedish and English systems, where those criminals who can be rehabilitated are sentenced in Sweden, and those who can't are sentenced by English courts to English jails.
PCR mixture (25 μl final volume) consisted of 1 × final concentration of Assay-On-Demand mix, 1 × final concentration of Universal PCR Master Mix Applied Biosystemss) and 20 ng cDNA.
The emergence of a first-rate tenor is always a cause for celebration, but Hymel's rare gift for singing the French "heroic" repertory — which demands a unique mixture of power, refinement, and technical flexibility in the highest vocal register — makes him doubly valuable.
However, rubber asphalt mixtures demand higher production temperatures than conventional asphalt binders due to the higher viscosity of asphalt rubber binder.
A further strong recommendation regards the need for the cooling down of the organic solution before its treatment with the acidic mixture demanded for the detritylation step, to avoid, or to reduce as much as possible any depurination of the product.
The results showed that by adding metakaolin at constant fluidity, the water demand of the fresh mixture increases with the increase of the metakaolin content.
Predictive analytics has become an important topic in expert and intelligent systems, with broad applications across various engineering and business domains, such as the prediction of exchange rate in finance, weather and demand for energy using mixture of experts.
