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We indeed found that leishmania exosomes contained RNA cargo which they were capable of delivering to host cells in vitro.
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The OMV content is delivered to host cells via either receptor-mediated endocytic pathway or the fusion with host cell plasma membranes [32], [33].
Several bacterial effector proteins delivered to host cells by the type III secretion system (TTSS) have been shown to suppress PTI [8], [9].
Moreover, exosome RNA cargo was delivered to host cell cytoplasm in vitro.
Based on the finding that CiaD is delivered to host cells, we postulated that CiaD acts as an effector protein.
We have found that CiaC is delivered to host cells and is necessary for the recruitment and activation of the Rho GTPase Rac1 [ 19, 27].
In this study we identified and characterized a novel C. jejuni effector protein that is secreted and delivered to host cells via the flagellar T3SS.
In our previous studies, we observed that leishmania exosomes were released into infected macrophages and were taken up by uninfected bystander cells, and that exosomal proteins were delivered to host macrophage cytoplasm [ 7].
The genome is then released from the capsid and delivered to the host cytoplasm.
It is caused by the protozoan parasite Trypanosoma cruzi, which is delivered to its host by kissing bugs, known formally as triatomines.
Bacterial induction of type I IFNs can be mediated through the TLR-dependent recognition of bacterial products, such as lipopolysaccharide (LPS), or through the TLR-independent recognition of bacterial ligands that are delivered to the host cytosol (Gonzalez-Navajas et al., 2012; Simmons et al., 2010).
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