In addition, Per1 and Per2 knockout mice showed higher number of implantation sites in comparison to WT mice and lower number of delivered offspring, suggesting embryonic losses [26].
Moreover, cynomolgus monkeys breed and deliver offspring throughout the year, which is consistent with human reproduction.
During the entire mating trial period (10 23 months of age), 9 of the 11 continuously AL-fed females became pregnant and delivered offspring at least once, with a total of 18 pregnancies and 54 offspring delivered.
Women who decreased their ICS medication have been shown to deliver offspring with lower mean birth weight and length than women who did not [ 13].
Between 10 and 12.5 months of age, 7 of 11 continuously AL-fed females became pregnant and delivered offspring (Fig. 3A).
In addition, we calculated for the experiments resulting in at least one delivered offspring the cloning efficiency as (3) delivered cloned offspring / SCNT embryos transferred As a benchmark for oocyte and donor cell quality, respectively, we also took the oocyte maturation rate, calculated asand the fusion rate, calculated asinto account.
In CR females returned to AL feeding at 15.5 months of age, approximately one-half remained fertile for 6 additional months and one-third continued to deliver offspring through 23 months of age.
In comparison, 82% of 8.5-11.5-month-old, 75% of 11.5-14.5-month-old, and 52% of 14.5-17.5 month-old BM-INF femachievedieved full-term pregnancies and delivered offspring.
This finding seems to be contradictory on a first view, but it has to be considered that the cloning efficiency was calculated only for cases in which the recipient delivered offspring.
By comparison, between 10 and 23 months of age, 8 of the 11 CR (10 15.5 months)/CR-then-AL-fed (15.5 23 months) females became pregnant and delivered offspring at least once, with a total of 30 pregnancies and 133 offspring delivered.
