The CD22-RTM NP delivers expression plasmids that encode CD22-RTM.
Some groups [ 44- 46] have reported that lentiviral vectors can efficiently deliver shRNA expression cassettes into a variety of cells with sustained long-term expression and without observable toxicity[ 47, 48].
RCAS avian viruses have been used to deliver oncogene expression and induce tumors in transgenic mice expressing the virus receptor TVA.
The pCAMBIA1304-MaPIP1 1-GFP construct and the pCAMBIA1304-MaPIP1 1-GFPembrane-loconstructandr were thensiently co-expressed in onion epidermal cells usedg asgene gun to deliver the expression plasmids (plasma00, BIO-RAD) [ 49].
Here we test the ability of the AAV1-F9 vector to deliver sustained expression and correction of factor IX (FIX) deficiency in genetically engineered hemophilic mice.
Then an NF-κB model [21] was appended to deliver mRNA expression.
The use of gene transfer to deliver an expression cassette to photoreceptors and RPE cells that produces a similar inhibitory molecule may be a less invasive strategy as it could produce a more sustained effect.
To deliver shRNA expression construct into NB cell lines, commercially available lentiviral shRNA vectors were used.
Virulence-attenuated strains of L. monocytogenes were also used to deliver eucaryotic expression plasmids into mammalian cells [ 19- 23].
The rice Act1 or maize Ubi1 promoters are constitutive promoters from plants that can deliver high expression of foreign genes in mononcots [ 5, 6].
