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The accurate experimental delineation of the structure of this clinically relevant gene represents an important step in facilitating ongoing drug development efforts.
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Reconstructed image stacks were created using XMReconstructor software (Carl Zeiss Microscopy GmbH) and the subsequent segmentation (delineation) of the structures of interest in the male and female genitalia was performed with Amira 5.4.5 (Visualization Science Group, FEI).
At the genome sequence level, the International Wheat Genome Sequencing Consortium IWGSCC, http://www.wheatgenome.org/) has tackled the delineation of the genome structure using the capacity to generate bacterial artificial chromosome (BAC) libraries from flow-sorted chromosome arms followed by sequencing of BACs that were arranged into minimum tiling paths (Feuillet et al. 2012).
Adding MAR in the reconstruction process eliminated the majority of intra- and extracorporal metal- and beam hardening artifacts, resulting in a better delineation of the neighboring structures and the jaw itself (Fig. 2b).
These results provide an indication of the added-value of this sequence resource in the framework of the delineation of gene structure, especially with respect to the determination of the transcription initiation site.
This work demonstrates the utility of genome scans for adaptive divergence in the delineation of stock structure, the traceability of individuals and products, and ultimately a role for population genomics in fisheries conservation.
However, an exact delineation of these structures is difficult due to considerable variability in shape and size across individuals.
The delineation of tooth structure from the surrounding alveolar and cortical bone was first determined on a slice-by-slice basis in the YZ (sagittal) plane (Figure 1) based upon visual inspection only.
In a highly sensitive dynamical model, such as the orbit-attitude CR3BP, periodic solutions allow delineation of the fundamental dynamical structures.
Magnetic resonance imaging (MRI) offers a subtle delineation of the soft-tissue structures anterior to the vertebral column, with the potential for more accurate and sensitive determination of PVST width.
In this context, it is reasonable to hypothesize that tumour cells by themselves, under the stimulus of the factors secreted, would contribute to the formation and delineation of the vascular channel structures and lacunae as had previously been proposed for other kinds of sarcoma [ 60, 61].
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