As MeDor outputs can be printed, saved, commented and modified further on, this offers a dynamic support for the analysis of protein sequences that is instrumental for delineating domains amenable to structural and functional studies.
Structure/function studies of rat SBP2 have delineated three domains: a putative regulatory domain comprising the N-terminal half of the protein (aa 1-407; humanumberingng is used throughout unless otherwise noted), a Sec incorporation domain (SID; aa 408-545) and an RNA binding domain (RBD, aa 623-784) [ 2, 3].
Since 1992, the CNC role in NSW, Australia has been delineated into domains of practice with functions attached to each domain.
Analysis of the deletion mutants delineated the domains of MP involved in the interaction with VPg and P10.
For a new protein to be compared against existing ones, delineating its domain boundaries is a non-trivial task for its own right (Holland et al., 2006).
By delineating the domain architecture of each protein involved in a fusion event, we are able to locate and filter those domains that appear to link an inordinate number of proteins together based on domain interaction graph connectivity analysis (see Methods).
We have previously shown a direct linkage between ERK2 and the cytoplasmic domain of β6, and have delineated the binding domain for ERK2 within the cytoplasmic tail of the β6-integrin subunit, EAE RSKAKWQTGTNPLYRG (the ERK2 binding sequence is italic) [ 16].
Tetraspanins compose a family of proteins with four transmembrane domains delineating two extracellular domains of unequal size.
We focus here on the phospholipid-binding properties of the major part of the central rod domain, namely, the sub-domain delineated by hinges 2 and 3 comprising 16 repeats ranging from repeat 4 to 19 (R4–19).
Co-IP analysis of lysates from the embryos expressing TBC1d24 mutants harbouring small deletions within the conserved TBC domain (delineated in Fig. 1c 50,51 indicated that the TBC1d24-ΔD mutant showed a significant reduction in the ability to bind Rab35, while the TBC1d24-ΔF mutant (which is unable to interact with ephrinB2) interaction was unchanged (Supplementary Fig. 3b).
