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While the inherent complexity of discrete neural circuits has proven difficult to decipher, many parallel methodological developments promise to help delineate the function and connectivity of molecularly defined neural circuits.
Thus the development of new isoenzyme-selective probes would be able to better delineate the function of a single isoenzyme and its individual contribution to the metabolism of a particular substrate.
To delineate the function of mir-148 and let-7b during lens regeneration we performed gain- and loss-of-function experiments.
Furthermore, the non-canonical Wnt/PCP pathway is not yet active at this stage and so we took advantage of these canonical Wnt/β-catenin-responsive cells to delineate the function of Nkd1 in antagonizing canonical Wnt/β-catenin signaling, hereafter referred to as Wnt signaling.
In our efforts to delineate the function of Rest during neural development, we generated several Rest deficient ES cell mutants to elucidate the role of Rest in the transition from pluripotent ES cells to multipotent NSCs and subsequently to mature neurons.
21, 22 The accumulating evidence on innate receptors has enabled us to delineate the function of BCG reagents.
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Site-directed mutagenesis and a comparative characterisation of the kinetic parameters, pH dependency of activity and thermal stability of mutant and wild-type enzymes have been used in association with crystallographic analysis to delineate the functions of several active site residues in a novel glycoside hydrolase family 8 xylanase.
We also use FARO to confirm and further delineate the functions of Arabidopsis MAP kinase 4 in disease and stress responses.
These results clearly delineate the functions of dSir2 in the two different metabolic tissues in affecting starvation survival.
Nevertheless, our findings might help delineate the functions of those genes that can be suppressed by HU from the functions of those that cannot, in the chromosome segregation pathway.
Further detailed investigations will be needed to delineate the functions of tsRNAs in leishmania biology, what roles they play in parasite-parasite, parasite-vector or parasite-host interactions, whether this involves their association with the host RNAi machinery and how they are targeted for exosomal packaging.
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