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To assess whether the four Neomys lineages corresponded to species, we used the Bayesian species delimitation program BPP version 2.2 [ 2].
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A crucial aspect that should be certainly taken into account in conservation programs is the delimitation of G. pyrenaicus into the four mitochondrial lineages found in this work.
To validate our morphological inspection, a multilocus coalescent species delimitation analysis was conducted using the program BPP [ 25, 34] with a reversible-jump MCMC method to estimate the posterior distribution for different species delimitation models.
On the other hand, the formal species delimitation test implemented in the BPP program allows posterior probabilities to be determined for one-species and two-species models successively applied to sister lineages within a phylogeny [ 2, 9].
We also studied the unsplittable feature of binary files; after specifying the delimitations through the metadata file, the program easily retrieves the correct input of the Map function.
Delimitation of these domains is, however, not consensual among the different software programs used for their determination [ 19, 20].
GMYC is a species delimitation method that provides an objective way to delimit genetic clusters [ 56].
We used the program Bayesian Phylogenetics and Phylogeography v2.0 (BPP, [ 15]) to perform species delimitation analysis.
Two recombination points were located by the program (at the left of positions 242 and at the left of position 582) in accordance with the delimitation indicated in Figure 4.
The delimitation of memory allowed a greater abundance of memories to flow in.
But Lauterpacht said Brandis's undertakings were "silent upon the availability of these very sensitive and confidential documents to those Australian officials involved in maritime delimitation matters".
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