Third, we examined spindle microtubule morphologies of dis1-deleted mutants by live imaging (note that dis1-deletion cells are cold sensitive for growth) [ 24].
However, when SOX2-deletion cells were transplanted into C57/Bl6 mice they remained tumor free [43].
Only the lentiviruses showed reduced infection efficiency in the BER deletion cells.
Furthermore, the rho3-deletion cells and apm1-deletion cells showed very similar phenotypic characteristics, including the sensitivity to the immunosuppressant FK506, the cell wall-damaging agent micafungin, Cl−, and valproic acid.
To test this notion, ctf7-203 smc3Q cells were crossed to ctf18 deletion cells and the resulting diploids sporulated.
Furthermore, rho3 deletion cells closely resemble apm1 deletion cells in terms of the presence of multi-lamellar Golgi, accumulation of abnormal Berkeley bodies, and vacuole fragmentation, which was observed by electron microscopy and drug and Cl− sensitivity.
Notably, electron microscopic observation of the rho3-deletion cells revealed the accumulation of abnormal Golgi-like structures, vacuole fragmentation, and accumulation of secretory vesicles; these phenotypes were very similar to those of the apm1-deletion cells.
There was no difference in HIV autointegration products between wild type and BER DNA glycosylase deletion cells.
Upon Rb deletion, cells escaped from cell cycle arrest and began replicating at levels equivalent to the original untreated cell population (Figure 2C, left panel).
We crossed rad61, ctf18 and elg1 deletion cells and sporulated the resulting heterozygous diploid strains to obtain the range of double and triple mutant cells.
