Therefore all discontinuous deletions are treated as continuous deletions in our analyses.
Thus, the screening for small deletions is carried out in a separate execution of the algorithm where a deletion is treated as a 'fifth base'.
To make deletions, we PCR-amplified the part of the plasmid, which should be left after deletion, DpnI treated the sample and ligated the resulting products after end-phosphorylation.
Incorporation of [35S]-methionine into total proteins and β-gal activity in the wild type and in the deletion strains treated with specific ETC inhibitors (sodium azide or CCCP) of ETC was assessed in the same manner as described above.
The difference in size corresponds to the exon IV/V deletion in treated animals.
In another small study, 18 AML patients ages 60 years and older, most of whom did not have the 5q deletion, were treated with sequential azacitidine and lenalidomide.
To identify the dose of diphtheria toxin that induced Foxp3+ Treg deletion, we treated Foxp3-GFP-DTR mice with 50, 10, 5 or 1 μg DTx per kg body weight and determined Foxp3+ Treg deletion after 24 h.
To induce widespread, acinar-specific Kras activation and/or Ptf1a deletion, we treated mice with three daily doses of tamoxifen at 0.25 mg/ml, a treatment paradigm that we found to drive widespread recombination (see below).
These mutants were also prone to single base pair deletions when treated with a DNA crosslinking agent, suggesting that menin promotes genome stability by several mechanisms [12].
Amplifications and deletions were treated separately in the analysis.
