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In this study, a long PCR strategy was used to rapidly genotype for the C4A deletion through specific primer design.
We developed an efficient and cost-effective PCR SNP assay designed for the screening of 22q11.2 deletion through consecutive homozygosity.
In the figure, gene amplification is denoted through a purple square and gene deletion through black squares.
(A) The HsD3B is a candidate gene for the Smith-Magenis syndrome, which involves chromosome deletion through recombination among repeats [30], [31].
The authors confirmed the presence of the heterozygous deletion through differential PCR and Sanger sequencing.
The authors find an APC promoter deletion through whole-genome sequencing and establish the deletion coordinates through Sanger sequencing.
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Dipeptides originated from sequential deletions through the generations were frequent (81%), but tetrapeptides arising from sequential additions were rare (1.4%) (Figure 2c).
The current study revealed both large (>1-kb, Fig. 1) and small (Table 2; Additional file 1: Table S1) deletions through alignment of sequences of 35 non-aflatoxigenic genotypes with the sequence of A. flavus AF13 (Ehrlich et al. 2005).
These Alus can mediate deletions through NAHR, but we have also shown that they may be involved in double strand breakage leading to NHEJ-associated mechanisms.
We also obtained dual-deletions through histidine deletion screening followed by regrowing overnight then 5-FOA and tryptophan screening.
To perform large precise deletions through TALENs, we tested the possibility of combining TALEN with ssODN.
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