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Unlike the mouse model, gene deletion technology (for example, gene knockout and knock-in) and trans-gene expression is also unavailable in case of guinea pigs.
It is important to introduce inducible gene deletion technology to highly regenerative systems like zebrafish, axolotl, and planarians, and we are actively working with others in the field on this.
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Thus, combining the gene-deletion technology with high spatial resolution QAR proved to be a valuable approach for studying the role of brain barriers in the regional distribution of drugs in the brain.
The effect of Smad7 on mouse development has been revealed by manipulation of Smad7 expression through transgenic overexpression, virus-mediated transfection and genetic deletion by knockout technology.
We appreciate this productive dialogue and an outside perspective revealing an important aspect of normalization that was not readily apparent to us when developing the Digital Deletion Detection (3D) technology.
A recent study in Saccharomyces cerevisiae used a comprehensive panel of yeast deletion mutants and microarray technology to facilitate the identification of the intracellular targets of a compound [1].
Taking advantage of the availability of a comprehensive set of variation from a fully sequenced genome, we have analyzed the performance of detecting insertion and deletion by a HTS technology.
As poor coverage can confound the identification of deletions, we sought to use microarray technology to confirm the deletions identified by the NGS data.
We excluded all the phenotypic annotations due to multiple gene KO experiments and only used those of null mutation homozygotes by target deletion or gene-trap technologies.
The yeast deletion project had a strong technology component.
The effects of gene deletion or insertion using transgene technology have also been studied in the bleomycin model.
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