sipD gene was deleted using one-step deletion strategy [59].
On average, we found that two more gene deletions would be required than the number of reaction deletions to completely eliminate the reactions suggested by OptKnock from the network (e.g. a four reaction deletion strategy would require deleting six genes).
In addition, items that still misfitted after the collapsing, were deleted using a step-wise top-down deletion strategy until the remaining items fit the model [ 83].
Using a targeted gene deletion strategy, we generated transgenic mice lacking the entire keratin multiprotein family.
We report a high-throughput genomic deletion strategy to screen for functional long non-coding RNAs (lncRNAs) that is based on a lentiviral paired-guide RNA (pgRNA) library.
The first strategy employs a Void Growth Model (VGM) to simulate the initiation of softening and the Hillerborg model for modeling of material softening, followed by an element deletion strategy that is developed in this framework.
To efficiently repair route structures, a node insertion and deletion strategy is proposed based on the average passenger demand for the direct services concerned.
Two independent clones of H3mm7 homozygous KO (H3mm7−/−) cells cells were constructed using the CRISPR/Cas9-system (see Supplementary Fig. 5a, b for the deletion strategy of H3mm7 in tetraploid C2C12 cells).
In this work we have engineered a new Nkx2-5 conditional knockout mouse in which flox sites flank the entire Nkx2-5 locus, and validated this line for the study of heart development, differentiation and disease using a full deletion strategy.
It is being relocated from one node to another using the immediate deletion strategy.
The deletion strategy is based on the overall contribution of each hidden unit to the network output.
