Sentence examples for deletion screening from inspiring English sources

SNP templates were generated by PCR using the same reaction mix and reaction profile used for homozygous deletion screening.

The 6TM family is better covered, partially through our efforts, but primarily through the efforts of individual laboratories also using PCR deletion screening strategies [see, for example, Wei et al. (2002)].

It should be noted that, as for genome-specific PCR and CAPS-based deletion screening techniques, this assay is not suitable for the detection of hemizygous individuals (those in which a specific locus is missing from one homologous chromosome but intact within the other) and is not amenable to sample pooling due to its reliance on detection of the lack of specific target sequence.

Zhu, S. et al. Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR Cas9 library.

However, although routinely performed in clinical workup [4] karyotyping, AZF deletion screening and CFTR sequencing elucidate the reason for infertility in only 5% of unselected and around 20% of azoospermic patients [1], [2].

From the 655 possible interacting genes identified through our deletion screening and mapping, we used gene ontogeny terms to select genes with possible functions in polarity, the cytoskeleton, membrane trafficking or signaling, as well as transmembrane proteins.

For some of these CNVs that are still rare in controls, such as the distal 16p11.2 deletions, screening a larger control group would help to ensure a more precise estimate of penetrance.

However, from the results of the nullisomic-tetrasomic lines in conjunction with the largest 5B and 5D long and short arm deletions screened, we could putatively assign TaPFT1-B to a location between 5BS FL 0.13 and 5BL FL 0.52; and TaPFT1-D to between 5DS FL 0.22 and 5DL FL 0.60, and these locations are consistent with the location determined for TaPFT1-A.

Systematic comparison of gene perturbation technologies: We have systematically compared the ability of genome-wide RNAi and CRISPR/Cas9 deletion screens to identify drug targets and essential genes, and have found important differences in their outputs.

3) Deletion screens in Drosophila: To detect novel genes important for cardiac function in the adult fly.

Deutschbauer et al. [19] performed an exhaustive heterozygous deletion screen in yeast.