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Finally, splicing inhibition and intron deletion reduce H3K4me3 levels and transcriptional output.
Both maternal diet and Cited2 deletion reduce embryo weight and kidney and thymus volume.
Let us call genes whose deletion reduce fitness potential by more than 3% essential, and the remaining genes nonessential.
Using "Essential Reactions Reporter", we found 135 reactions to be essential as their deletion reduce the flux of biomass significantly.
This workflow may predict some interesting pairs of reactions whose simultaneous deletion reduce the biomass, which otherwise are found to be non-essential during single-reaction deletion analysis.
As expected, this is not related to general transcription factors, whose deletion reduce expression level, but to many specific chromatin regulators and, notably, to histones.
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In the Langdon allele, the 7.2 kb deletion reduces intron size and deletes TE fragments, thus reducing both intron size (therefore likely facilitating splicing) and probability for cryptic promoters.
CD177 deletion reduced neutrophil counts in inflammatory skin caused by S. aureus.
Single NPY or dynorphin deletion reduces food intake or increases fat loss.
Moreover, NEDP1 deletion reduced PCNA-polη interaction, thus prevented TLS pathway and increased cell sensitivity to H2O2-induced oxidative stress (Figs. 6B and 7A).
In addition, Ubc12 knockout enhanced PCNA-polη interaction, but NEDD8 expression or NEDP1 deletion reduced the recruitment of polη (Fig. 6), indicating that enhanced NEDDylation reduces excessive polη staying on the replication fork to avoid high rate of mutagenesis.
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