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The idea is to make it easy for any Uber employee to build deletion into new projects, so that unnecessary data retention isn't an issue for Uber going forward.
To overcome these complexities, we also separate deletion into two components.
A 200 bp dsDNA substrate designed to introduce a 1,173 bp deletion into the Giles lysA gene (31) was co-electroporated with Giles phage DNA.
No differences in arabinose utilization were detected on either MacConkey plates containing only arabinose or on minimal media containing arabinose as the sole carbon source, and therefore we did not introduce the ΔaraBAD23 deletion into the S. Typhi Ty2 lineage.
We also investigated the role of LevQ in the altered expression of the levD operon caused by the C-terminal truncation of LevT by mobilizing a levQ (sp) deletion into a levTΔC (em) genetic background.
In order to estimate the lower limit of aCGH to detect copy number changes among samples, we set up an experiment in which we diluted DNA from an individual carrying a deletion of known size at 7q11.23 (typical Williams-Beuren Syndrome deletion) into a pool of normal DNA.
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Site-specific nucleases create DNA double stranded breaks which, during repair, can be used to introduce site-specific point mutations, insertions and deletions into the plant genome.
Deletions into the 3′-NTR, up to 66 nts from the 5′ direction and 11 nts from the 3′ direction, deleting or disrupting putative SL1, SL2 and SL3, or SL10, resulted in continued replication, suggesting that these sequences are not essential for basal-level replication, but are required for efficient replication.
We then took the more extreme approach of introducing large deletions into the HC.
We set out to identify region(s) of gemin5 that are required for association with cap-sepharose by introducing serial deletions into the FLAG-tagged gemin5 cDNA construct.
In particular, methods have been developed to introduce deletions into the yeast genome using PCR fragments.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com