We used decreasing levels of galactose to assess whether the deletion factors had any effects on growth that could be missed due to the lack of sensitivity on media containing 2% galactose.
The deletion of factor Xa could reduce the network efficiency greatly, from 17.822 to 8.894.
The deletion mutant (factor VΔ680−709) was constructed with the same kit.
In contrast, deletion of factors with other roles at defective replication forks, such as Top3 or Rad51, did not cause synthetic lethality with cdc48-3.
Also, supporting this are studies demonstrating that deletion of factors involved in resolving oxidative base damage, such as Ogg1 and Nth1, results in telomeric abnormalities including fragile telomeres and increased telomeric DSB formation (Wang et al., 2010; Vallabhaneni et al., 2013).
Serial deletion of transcription factors revealed several factor combinations that efficiently induced GFP expression.
1) writes in a write-back cache, including periodic write-back and deletions, important factors in file system cache performance.
In the other deletions, α-factor signaling was blocked giving rise to morphologies and transcriptional activation comparable to AI-Ste12p in the mfα2Δ strain (Table 1, Figure 5 and S2).
The OptORF approach can also suggest transcription factor deletion as an alternative to metabolic gene deletion or overexpression, which provides greater flexibility in metabolic engineering strategies.
This analysis suggested that 11 items be deleted: these were compared with the 18 items suggested for deletion by the factor analysis of the 32-item MLCDP (Table 8).
