Sentence examples for deletion boundaries from inspiring English sources

These latter two deletions [129], [130] have only been characterized cytogenetically, which is a relatively imprecise method [131], and it would be beneficial to map the exact deletion boundaries to confirm whether the deleted regions encompass DIA1.

To determine the deletion boundaries, PCR products were purified using ExoSAP-IT® (USB, Cleveland, USA).

All deletion boundaries or PCR generated constructs were verified by sequencing.

In order to determine the deletion boundaries, PCR products were purified using ExoSAP-IT® (USB, Cleveland, USA).

New ΔmtDNA variants (containing deletion boundaries that differed from ΔmtDNA-C) were detected in 19 different experimental C. briggsae lineages.

The microhomologies observed at the deletion boundaries ranged from 1 bp to 11 bp in size (see Additional file 1).

Deletions ≥250 bp were detected using combined paired read data and localised homology mapping across the deletion boundary.

It is expected that only one or two pairs of PCR reactions will produce specific PCR products spanning the deletion boundary, since the other primer pairs should be too far from the breakpoint for efficient amplification.

PennCNV combined with visual evaluation of images generated by the Beadstudio program was used to determine deletion boundary definition of sufficient precision to discern independent events, with near-perfect concordance between parent and child in about 98% of the 399 events detected in the offspring; the remaining 7 deletions were considered de novo.

Fig. 2 demonstrates amplification of several breakpoints scattered by the deletion boundary in the range of 15 bps in one PCR reaction.

In each case it was possible to identify a probable deletion boundary that overlapped with a repetitive genomic element found duplicated at the boundaries of the parent genomic structure.