This mutant protein is slightly less susceptible to calpain cleavage and its deletion blocks calpain cleavage (Fig. 5E,F), further demonstrating that this region of the protein is important for cleavage (Fig. 2E).
Our results demonstrate that Gsk3 deletion blocks radial migration and that Gsk3 over-expression may enhance it.
GSDMD deletion blocked pyroposis and IL-1β production induced by all the stimuli tested (Supplementary information, Figure S3A-S3C).
Both caspase-1 deletion and GSDMD deletion blocked pyroptosis (shown by PI-positive cells), but the morphology of the cells still changed to be apoptotic-like.
In a similar way, fructose-bisphosphate aldolase (Ot10g01490) deletion blocks starch synthesis during the day as it prevents the input of CO2 to the pathway.
Because Smg6 deletion blocked stem cell differentiation, somatic cells could not be obtained, thus providing a fitting explanation for the cause of embryonic lethality.
Note that all deletion blocks (with respect to S) in T are ignored as they contain no information to be transmitted about T.
The fact that Smg6∆/∆ ESCs are viable and Smg6∆/∆ embryos die soon after implantation raised the possibility that Smg6 deletion blocks ESC differentiation.
Ectopic NFATc1 expression alone in osteoclast precursors is sufficient to produce mature osteoclasts, whereas NFATc1 deletion blocks the ability of the precursors to differentiate into osteoclasts (Takayanagi et al., 2002).
Similar to the knockout of Nlrp3, GSDMD deletion blocked the release of p20 caspase-1 to the culture medium while the knockouts of other seven genes had no effect, suggesting that GSDMD is involved in inflammasome activation.
