This difference in the extent of deletion between CD4 and CD8 with CD4s being deleted significantly more extensively suggests that peripheral deletion of CD4 cells also took place, in addition to central deletion occurring for both CD4 and CD8.
In cell line WHCO1 the probe mapping at 3p22 was deleted, putting the telomeric boundary of the deletion between 3p25 and 3p22.
Recombination suppression in this region was associated with an inversion and large deletion, between markers 101O9L and 14A11-71K, in japonica relative to submergence intolerant Teqing and tolerant IR40931-26.
Sequence analysis of the deletion transformant confirmed that the chromosomal INU1 locus harbored a 2565-bp deletion between the gRNA-2 and gRNA-1 targeting sites, yielding Δinu1 (Km02-063) strain (Fig. 1a).
The evolution of the strain was associated to a deletion between GPD1 and GPP2, resulting in the production of a fusion protein with both glycerol-3-P dehydrogenase and glycerol-3-P phosphatase activities.
In the TP0132, a false 1 nt deletion between coordinates 153123 153124 was found.
The Gabrb3 and Ube3a double targeted mutations without a deletion between them were also obtained in germline (data not shown).
Moreover, in the published SS14 genome [27], a false 1 nt deletion between coordinates 1124003 1124004 of the Nichols genome (i.e. between SS14 coordinates 1125634 1125636) was found.
In the general case, deletion between two different, asymmetric sites might require up to four ZFRs, each with DNA-binding specificity for one half-site.
The sequences flanking the breakpoint are: GAATACTTTTGTG and TATTACACTTTT, corresponding to a 1334 base pair deletion between nucleotides 13646 and 14969 in cosmid T19A5.
We therefore made a null mutant of caps by targeted recombination induced deletion between two piggyBac elements (see Materials and Methods).
