We describe a machine learning method to distinguish genomic regions where breakpoints would be expected to have deleterious effects (called breakpoint-refractory regions) from those where they are expected to be neutral (called breakpoint-susceptible regions).
Their ecological worth in the face of conflicting and deleterious demands calls for effective conservation and protection from undue human impacts.
SIFT was originally validated upon previously published large-scale mutagenesis experiments [26] [29], so we used an identical dataset to validate whether or not B-SIFT could call deleterious mutants at a rate similar to that of the original SIFT.
Unusual donor sites (i.e. with cytosine at position +2) are detected by information analysis, but could be falsely called deleterious by consensus sequence-based methods.
Lead poisoning, also called plumbism, deleterious effect of a gradual accumulation of lead in body tissues, as a result of repeated exposure to lead-containing substances.
SNVs predicted to be damaging: "DM" in HGMD (Human Gene Mutation Database Professional, version 2013.3), missense SNVs called as "deleterious" by SIFT, "probably damaging" by PolyPhen2, grantham score above 100 or positions with a phyloP value above 2.5.
We used cutoffs of SIFT <0.05 and B-SIFT >0.5 to call a variant deleterious or beneficial, respectively.
The steady accumulation of these deleterious mutations is a process called Muller's ratchet [6], [11], [12].
However, because of its complex sexual life cycle, yeast can filter out these deleterious mutations by a process called 'genome renewal' (Mortimer et al., 1994; Mortimer, 2000; Wang et al., 2012b).
Conclusions: These results demonstrate in an in vivo animal model that lowering the dose-rate suppresses the potentially deleterious impact of radiation and calls attention to the need for a deeper understanding of the biological impact of low dose-rate radiation.
The absence of recombination between proto-X and proto-Y led to the gradual degeneration of the Y chromosome by accumulation of deleterious mutations in an irreversible manner (called Muller's ratchet) or through hitchhiking by favorable mutations (called selective sweeps) [ 55].
