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ORFs that were specifically deleted in the library strains used for this project are shown in Figure 4E (left column) along with the overlapping ORFs that were partially deleted (right column).
When overlapping occurs, it is not completely certain that loss of the gene that was precisely deleted in the library mutant (from its start codon to its stop codon) has caused the observed phenotype.
Among the 15 other loci, 5 verified genes that were completely deleted in the library strains (EAF1, GND1, RSM7, TSR2 and VPH2) overlapped unverified ORFs and 10 unverified ORFs whose coding regions were deleted overlapped verified genes (NET1, RPL39, RPC53, YKL075C, RPL12B, NPL3, MDM34, TOP3, SPT5, and UBX2).
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These experiments supported the annotation of YBR134w as a dubious ORF and revealed that HSL7 was not deleted in the commercial library (see Materials and Methods).
That provision has been deleted in the new guidelines.
PTK2 was never deleted in the primary tumors and deleted in only 2/18 cell lines (11%).
Sequence contamination of lambda-ZAP II vector or lambda phage (87 clones) and the host organism Escherichia coli (130 clones) were deleted from the libraries based on in-house BLAST searches.
I spent many hours there as a teenager floating up and down the strangely narrow escalators, padding about the thick, orange carpets and finding rare and deleted vinyl in the music library.
Drag the encoded file(s) out of your iTunes library and then delete the library's copy.
The CA tweet was deleted in around half an hour.
For completeness, the analysis included verified genes that were partially deleted when an unverified gene was precisely deleted during creation of the library.
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