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According to linear prediction theory, the delayed sample points in the time domain correspond to the array sensors in the spatial domain.
In this regard, a hypoxia-associated gene expression signature was detected in peripheral blood mononuclear cells (PBMCs) after delayed sample processing compared to immediate sample processing [ 20].
However, white blood cell isolation kits can induce a technical bias that can confound the original expression profile of the samples and delayed sample processing can affect gene expression profiles [ 13, 14].
Therefore, the low glucose concentrations are most likely related to a delayed sample analyses, because it is well known that glucose concentrations rapidly decrease in blood samples (6 mg/dl per hour at room temperature)[ 19].
We also performed an analysis restricted to participants swabbed within 4 days of illness onset because of potential misclassification bias with delayed sample collection.
Our results indicated that RNA later enables long-term tissue preservation for RNA and protein extraction compatible with delayed sample return from flight.
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In this line of thought we can think in concentrating the delayed samples into a finite number of points that somehow "average" a given set number of sampling instants (see Figure 1).
The delayed samples (6%) are not discarded, but the delay is registered in the database.
In addition, we may have missed the peak levels of the measured inflammatory markers by the delayed sampling time point.
This low percentage of horses with seroconversion is probably the result of the delayed sampling, which was initiated only 12 days after the index case.
Finally, 3 and 6 h control samples were compared to the 3 and 6 h delayed samples, and also 3 and 6 h smoke-treated samples were compared to the 3 and 6 h delayed samples with 3 technical replicates, totalling 12 slides.
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