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Fruits from such lines exhibited delayed ripening and extended shelf life for ∼45 days, with improved juice quality.
These results suggest that the down-regulation of ACS homologs using RNAi can be an effective approach for obtaining delayed ripening with longer shelf life and an enhanced processing quality of tomato fruits.
Phenotypic analysis of the G194D mutant fruit showed complete delayed ripening and yellowing with improved shelf life and, as predicted, the L124F mutation did not have an effect.
Suppression of MADS8/9 causes delayed ripening; one line MADS8/9as is particularly severe.
However, purple fruit exhibit delayed ripening after breaker compared to red fruit.
Although this is the case, suppression of ACO results in reduced ethylene and delayed ripening in a number of fruit species including tomato, melon, apple, papaya and kiwifruit.
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Even in species where endogenous ethylene seems to play little if any role as an endogenous regulator, exogenous ethylene will often promote ripening characteristics and can be the target of post-harvest strategies designed to accelerate, synchronize or delay ripening.
The optimized coating formulation (5 g/100 mL AG, 1 g/100 mL SC and 2.5 mL/100 mL TE) maintained suitable internal gas composition to delay ripening, showed higher overall acceptability and shelf-life of 7 days at 28 ± 2 °C compared to 4 days of control.
Clay and limestone based soils are often cooler, allowing less heat to reach the vines, delaying ripening.
A wide list of approaches, ranging from the application of growth regulators to delay ripening, fruit storage in controlled atmospheres and breeding or genetic engineering of leader lines have been used to control fruit ripening and softening.
During the earlier phases of berry development auxin and cytokinins may act to delay ripening [ 6].
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