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Overexpression of Rab14 dominant negative mutants delayed inclusion enlargement, and impaired bacteria replication as determined by IFU.
In agreement, our results show a delayed inclusion enlargement, an impaired bacteria replication and the generation of abnormal bacterial forms in infected cells with reduced sphingolipid acquisition due to the overexpression of the negative mutants of Rab14.
Interruption of this trafficking pathway by overexpression of the negative cytosolic mutant of Rab14 impaired lipid uptake resulting in a delayed inclusion growth, an abnormal chlamydial developmental cycle and an impaired bacteria replication.
Another consequence of this delayed inclusion process is that we decided to stop inclusions as only 601 out of the 1000 expected households were included.
Indeed, DNA material for 31 patients was not available either because of patient refusal to participate in the genomic study or because of delayed inclusion of the last patients.
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Thus, a 4-hour observation period seems to be a good compromise, allowing evaluation of the patient's clinical condition and permitting stabilization, without delaying inclusion in a therapeutic trial.
These guarantees can be enforced, for example, based on data size, number of hits in a certain data store object, or delay inclusion.
In addition, our results show that Rab14 is required for chlamydial inclusion growth and development, since the presence of its negative cytosolic mutant delays inclusion enlargement.
In the meantime the airline industry should stop fighting to delay its inclusion in a global emissions-trading scheme.The Gulf airlines, already criticised by rivals who will be hit harder by the Europe-only scheme that comes into effect in 2012, should take a lead by pressing for a similar carbon-trading scheme covering their own region.
For it's a kind of lexical Catch-22: since editors at most traditional dictionaries won't include a word until they see published evidence of its use, holding off on using a word just because it's not in the dictionary can actually delay its inclusion.
They also apologized to Billups and his family, and said that his inclusion delayed the deal.
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