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Given the function of mTOR pathway to regulate protein translation, our results strongly suggest that reduced cell proliferation is due to lowered expression and impaired activation of Akt, and that delayed expression of mTOR subsequently causes delayed expression of Jak2 and STAT5, which are crucial for DC development and functions.
Despite the rapid transcription of the miR-146a and miR-146b genes, delayed expression of mature microRNAs implies inefficient or delayed processing of miR-146a/b in cytokine-stimulated endothelial cells.
Applying force to a delayed expression causes the expression to be evaluated in the environment from when it was delayed.
A detailed expression profiling during the panicle development of O. glaberrima and O. barthii using qRT-PCRs and in situ hybridization, confirmed a delayed expression of the phased siRNAs as well as their lncRNA precursors and regulators (miR2118 and MEL1 gene) in O. glaberrima compared to O. barthii.
We have found that TPA induces delayed expression of K-RTA, comparing to butyrate or TPA plus butyrate (Fig. 6B).
Similarly we observed reduced and delayed expression of myogenin in cultures of α-syntrophin siRNA treated C2 cells.
It was recently reported that signalling through the ERK MAPK delayed expression of muscle-specific miRNAs in C2C12 myoblasts undergoing differentiation [11].
Thus comparing to butyrate or TPA plus butyrate, treatment with TPA induces delayed expression of K-RTA RNA in BC1 cells.
The latter showed delayed expression profiles when compared to relapse-free patients: in these patients expression imbalance was reverted later in the pregnancy, i.e. at sixth month.
In comparison, there is delayed expression of some β, and most of the γ-crystallins in the K6W-Ub lens (Figure 3E G).
The replicon based system showed a delayed expression profile compared to the pEGFP-N1 driven expression, transfection efficiency of the influenza replicon system was approximately 50% lower (Figure 3).
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