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We observed that FRG1 overexpressing cells exhibited a consistent 1 2 hour delay in exit from G1 and entry into S-phase (Figure 4B & Table 2).
Neither I3C alone, nor in combination with putrescine caused a delay in exit of cells from G2/M, however, cells in both treatment groups appeared to accumulate in G2/M by 32 hours after release (Fig 6B).
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If she cannot persuade lawmakers to accept her plan, Mrs. May will get a shorter delay in exiting the European Union — until April 12.
This growth lag was seen as a delay in exiting stationary phase in the ΔdPGM strain relative to ΔiPGM and MG1655.
We created PGM null mutants in E. coli and discovered the ΔdPGM mutant grew slowly due to a delay in exiting stationary phase.
HPr mutants, in both wild type and ∆ crh background, displayed a prominent delay in exiting the ripening period (Additional file 17: Figure S10A).
As has been suggested for separase function in cytokinesis [ 35] an indirect mechanism via Cdk1 inhibition could conceivably be at work, and indeed separase RNAi embryos suffer extensive delays in exit from meiosis [ 12].
RICHARD JACOBSON Charlotte, N.C., Nov. 1, 2011 To the Editor: I find the delays in exiting the plane even more annoying than the delays in boarding.
It has been recently published that cycling extracts forced to use a mutant cdk1AF (cdk1AF; thr 14>ala, and tyr 15>phe), refractory to inhibitory phosphorylation, enter mitosis with slower kinetics than control extracts and are delayed in exiting mitosis [17].
Rac1 is required in cycling progenitors, since in its absence they are significantly delayed in exiting the cell cycle, probably due to a longer G1 phase.
The official termination of the hugely expensive e-borders programme this year lies behind the delay in imposing exit checks.
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