The tissue blocks were dehydrated for 5 days in 70% ethanol, then 2 days in 80% ethanol, and finally 1 week in 96% ethanol.
The fungus wiped out all colonies dehydrated for 14 days or less, but 60% of those dehydrated for 21 days remained completely free from infection.
Some of the samples were fixed in 4% paraformaldehyde in PBS at 4°C for 2 days, dehydrated by immersion in 0.25% sucrose in PBS at 4°C for 2 days, and then dehydrated in 0.4% sucrose in PBS at 4°C for 4 days.
Late clutches have poor survival rates, possibly due to chicks getting dehydrated on hot days as the year progresses or being eaten by wedge-tailed eagles.
Rat kidneys were fixed with 4% paraformaldehyde for 2 days, dehydrated with ethanol and xylene, and embedded in paraffin.
Specimens were fixed in 70% ethanol for 3 5 days, dehydrated in increasing concentrations of ethanol, cleared in xylene, and embedded undecalcified in methyl methacrylate.
The bones were fixed in 10% normal buffered formaldehyde for 5 days, dehydrated by ascending concentrations of ethanol and finally embedded in methylmethacrylate (Technovit 7200, Heraeus-Kulzer GmbH, Wehrheim, Germany).
Fish were then fixed in either Bouin's fixative (750 ml picric acid, 250 ml 37 40% formalin and 50 ml acetic acid) or 10% neutral buffered formalin (Sigma) for at least 2 days, dehydrated through a series of graded ethanol solutions, washed in clearing agent (Fisher Scientific) and embedded in paraffin blocks.
The collected osteochondral samples from the intermediate region of the medial femoral condyles were fixed with 10%% neutral formalin for 24 h, decalcified with 5%% nitric acid for 4 days, dehydrated through a series of graded ethanol solutions, cleared in xylene, and embedded in paraffin.
Drink lots of water, its easy to get dehydrated on hot days.
