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Representative histologic examples with differing degrees of staining are shown in Fig. 2.
To determine the intrapartum and perinatal results associated with different degrees of staining of meconium stained amniotic fluid (MSAF).
In cases where different degrees of staining were observed within the same section, average values were taken e.g. 2+/1+ = 1.5.
Five grades distinguished the degrees of staining, which represented five reaction coefficients, respectively.
The immunostained sections were graded semi-quantitatively for degrees of staining intensity (negative, 0; positive, 1+ to 3+) by neuropathologists blinded to clinical information.
In general, all degrees of staining were recorded but, where only overexpression was considered relevant, positive scoring was restricted to those tumour cells showing staining intensity above that of the adjacent benign epithelial cells.
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The extension of staining in each cancer specimen (i.e., the percentage of tumor cells displaying any degree of staining) was rated as absent (no stained cells); limited (≤ 35% cells); moderate (36%69%%); or extensive (70%100%%).
On the other hand, the degree of staining was markedly reduced in tissue sections by morphine.
Immunohistochemically, there was a difference in the degree of staining for HMB-45 between primary and metastatic lesion.
With postconditioning, the spatiotemporal pattern of TNC deposition came to resemble that of control animals, while the degree of staining became weaker (Figure 4). Figure 4 Immunostaining for TNC at 3, 7, and 14 days after reperfusion with and without postconditioning is presented.
Tumor cells not showing any degree of staining were counted as negative.
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