The less stable the hybridization is, the more quickly the DNA strands will dissociate when heated; hence, low DNA melting temperatures typically suggest low degrees of sequence similarity.
The encoded proteins, named BFL-1, BFL-2 and BML, exhibit high degrees of sequence identities with Viperidae snake venom saccharide-binding lectins (around 60% with Trimeresurus stejnegeri venom lectin, Crotalus atrox venom lectin and Agkistrodon piscivorus venom lectin).
In doing such a test, we first noted that the Phasianidae mtDNA D-loop region was subjected to different degrees of sequence conservation.
Fig. 1 shows the upstream part of the gene, divided into regions with varying degrees of sequence similarity relative to other PgiC sequences.
We sought to decode conserved features within each fold family despite the vast degrees of sequence divergence, so as to better understand the factors governing the protein fold code.
Although some dominant response patterns may be due to degrees of sequence conservation, recent analyses show that subdominant responses outside of highly conserved protein regions can significantly contribute to the in vivo control [13], [14].
One of the most remarkable findings of these studies is that relatively small degrees of sequence diversity, e.g., 10 different random sequence for esterase activity, are sufficient to allow Darwinian selection of random polypeptides composed of about 140 amino acid residues [17].
BLASTP analysis of the identified proteins also revealed high degrees of sequence similarities to a recently characterized fungal β-CA from the pathogenic yeast C. albicans, two CAs of the pathogenic basidiomycete Cryptococcus neoformans as well as to non-characterized homologues from other ascomycetes and the zygomycete Rhizopus oryzae (Figure 1A+B).
For example, TIR gene family members display varying degrees of sequence homology.
Consequently, some satDNAs share high degrees of sequence similarity to parts of the original TEs.
Captured genes exhibit varying degrees of sequence similarity to their wild-type progenitors.
