Figure 4 Calibration of the degree of rescue of transgenic flowers.
To quantify the degree of rescue, we divided the total area of deep ElaV positive expression by the total area of the differentiated eye epithelium.
To quantify the degree of rescue, each hemisegment was scored as good rescue, mild rescue or weak rescue as shown in Figure 3B and S3A D (see Figure 3B legend for details on each category).
Co-treatment with mannose rescued 2-DG's inhibition of LLO synthesis at lower (0.6 mM), and higher (3 mM) concentrations of 2-DG, however the degree of rescue was less prominent at the higher 2-DG concentration.
The degree of degeneration is similar to that in the Trα−/− background, suggesting that the degree of rescue is contributed by removing transducin signaling and not by formation of Q344ter/Arr1 complex in the ONL and inner segment compartments.
Quantification of actin tail formation also revealed a similar degree of rescue (Fig. 2C).
The degree of rescue is ligand-related and is associated with activation of specific signaling pathways.
The degree of rescue of the dystrophic phenotype was determined as described previously (18), and the severity of the muscle phenotype by counting the number of damaged somites.
The degree of rescue generally correlated positively with AMOT p130 re-expression levels, and peaked at levels that approximated those of the native endogenous protein.
We take the reviewer's point, and have modified the sentence as follows: "the degree of rescue generally correlated positively with AMOT p130 re-expression levels".
