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These data were consistent with observations made using Affymetrix micorarrays (table 3) and correlated with the different degrees of induction of both IGF2 and H19 transcripts as quantified by real time PCR, ranging from 60 80 fold (batch 1and 2) to 600 700 fold (batch 4).
Although all genes modulated in the α-particle exposed WBCs were also observed after X-ray exposure, there were differing degrees of induction.
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The degree of induction was evaluated by detecting insulin-producing cells.
The number of DEGs and their degree of induction were greater after the 7-day SiAF treatment than the 7-day SiNF treatment (Table 1).
The degree of induction by the 7-day SiAF treatment was greater than that by the 1-day SiAF treatment or the 7-day SiNF treatment (Additional file 3: Table S2).
The genes required for ET synthesis were also induced in plants treated with either SiAF or SiNF for 7-days, and the degree of induction by SiAF treatment was again higher than that by SiNF treatment (Figure 3b).
Moreover, the expression of germin-encoding genes was also activated by the 1- and 7-day SiNF treatment; however, the number of induced genes and the degree of induction in the 7-day SiNF-treated plants was less than those in the 7-day SiAF-treated plants.
The degree of induction for the interferon signaling-related subset A is also reduced, albeit to a lesser extent.
The ASO tests showed an unexpected observation of a significant degree of induction of TLR-related genes by one of ASOs designed for the knockdown of the Gαi2 gene.
A similar correlation in the degree of induction of HMGCR and LDLR alternative splicing was seen after sterol loading in 31 lymphoblast cell lines incubated with 1 µg/ml 25-hydroxycholesterol.
The only difference was that the degree of induction of each gene was 1.25 (DHH) to more than 10 (IGFBP3) times greater in cells expressing the AML-associated fusion (Table 3).
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