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Their results indicated an inability to carry on viable pregnancies and some degree of embryo lethality in these mice.
In addition, seed fertility and degree of embryo lethality of the M1 plants were estimated as previously described [ 9] with the following modifications.
Although patients diagnosed with polycystic ovary syndrome (PCOS) often have a large number of oocytes available for insemination, their overall quality is poor as determined by the degree of embryo fragmentation and miscarriage rate (reviewed by Qiao and Feng, 2011).
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The follicular levels of NO were associated with fragmentation degree of embryos (p=0.030) in IVF cycles.
Note that we can detect relatively small changes in stiffness using this approach (20%), despite the high degree of embryo-to-embryo variation in tissue mechanical properties [43].
Seed fertility of M1 plants and the degree of M2 embryo lethality was quantified by estimating the proportion of fully or nearly sterile fruits (classes As and Aa) and the proportion of semi or normal fertile fruits (classes B and C).
Although the embryonic tissue is certainly complex and non-linear, simple models with only a few measurable parameters may be more useful than complex models given the high degree of variability of embryo mechanical properties [43], [59], [60], [66], [67], [68], [69].
Since females in any given population are not synchronized with regard to gestational stage, it could be misleading to compare populations using GSI as a singular measure of reproductive health or fecundity (as is a common practice in the piscine literature) without knowing the gestational stage or degree of tradeoff between embryo size and number.
For the morphological score, the degree of fragmentation of the embryo and the uniformity of the blastomeres were assessed daily.
Even though the parameters of this optimization are far from being mastered, it has been clearly shown that specific culture media generate a lower degree of stress for the embryo [ 5, 70].
One possible explanation might be a variation in growth conditions, which could lead to a higher degree of stress on developing embryos in the experiments by Deprost et al. The fact that these authors observed some embryo arrest in wild type control lines supports this possibility.
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