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Cultures were viewed using an inverted microscope to assess the degree of confluence and the absence of bacterial and fungal contamination.
In addition to vacuum, the rate of desiccation, the temperature at which cells are maintained, the degree of confluence when dried, and the presence or absence of light have a large effect on the ability to retain viability in the desiccated state.
The extent to which the issue is front and center can be best understood by watching the spectacle of the upcoming elections in the United States and by delving in to see that, despite the seemingly huge chasm between a Donald Trump and a Hillary Clinton, this is an issue where they, in fact, have some degree of confluence...in cause and outcome.
Drusen size, number, and degree of confluence are significant risk factors for the development of AMD (Bressler et al 1990).
Passage 11 MSCs from each age group were harvested at the same degree of confluence using identical procedures.
It is not clear whether the cells are tested in isolated conditions (like in Fig 2B) or with some degree of confluence (like in Fig 2E).
Similar(47)
Immunocytochemistry was performed at various degrees of confluence to ensure that there were no changes in gene expression resulting from cell density (data not shown).
Differences in cytotoxic effects can be explained by different degrees of confluence because confluent cells are more resistant to toxic damage than cells in the log phase [ 30, 31].
A previous study of hybrids generated between mouse thymocytes or fibroblasts fused with mouse ESCs that had been grown to different degrees of confluence (Sullivan et al., 2006) had suggested that reprogramming could be optimized using stem cells enriched for G2/M phases of the cell cycle.
This increased expression of NPHP1 and NPHP4 during cell polarization is consistent with the dynamic subcellular localization of these proteins according to the degree of epithelial confluence.
Upon Cetrorelix treatment, not only did the levels increase, but also the molecules were redistributed to the cells' periphery; this was particularly evident at sites of cell cell contacts, regardless of the degree of cell confluence.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com