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Our analysis focused on to what degree nucleotide fixation was caused by artificial selection during soybean domestication.
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Here, we use a cysteine engineering approach to create a high resolution, FRET-based sensor that reports a large, ∼70 degree nucleotide-dependent angle change of the light-chain binding region.
Based on the alignment of sequences of the bont/B gene (3876 bp) present in the GenBank database, the region encompassing nucleotides 920 to 3727 was selected for PCR amplification because it has shown a certain degree of nucleotide polymorphism.
To find out the degree of nucleotide polymorphism between the PWD/Ph and C57BL/6J mouse strains, we aligned 73 uniquely mapped BESs (32,182 nucleotides) with their C57BL/6J genomic counterparts and found 297 single nucleotide substitutions.
Goat TLR 1 10 gene sequences revealed a high degree of nucleotide identity with sheep and cattle sequences (>90%) and 75 85% with pig, mouse and human sequences.
This high degree of nucleotide sequence conservation has permitted the design of primers complementary to such sites facilitating "shotgun" cloning of skin or skin secretion-derived cDNA libraries from hitherto unstudied species.
Thus the "shotgun" cloning approach described can exploit the unusually high degree of nucleotide conservation in signal peptide-encoding domains of amphibian defensive skin secretion peptide precursor-encoding cDNAs to rapidly expedite the discovery of novel and functional defensive peptides in a manner that circumvents specimen sacrifice without compromising robustness of data.
The degree of nucleotide polymorphism found between grain and sweet sorghum was in the order of one SNP per 248 base pairs, with chromosome 8 being highly polymorphic.
The APMV-5 leader has a high degree of nucleotide sequence identity with those of the other APMVs (Fig. 2a).
The degree of nucleotide variability (v) at each position of the 28-mer was defined as v = 1 f, where f is the frequency of the most frequent nucleotide.
Clonal relationships established via goeBURST are based on the character state of the housekeeping gene allele, and do not take into account the degree of nucleotide sequence heterogeneity.
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