A possible explanation for this is that the compounds do not dissolve in ethanol but are instead degraded in solution.
Because sodium pyruvate rapidly degrades in solution, a simple derivative of pyruvic acid, namely ethyl pyruvate, has been investigated as a therapeutic agent in preclinical studies.
Penicillins were degraded in solution by reaction with 0.1 N sodium hydroxide and with 0.1 N hydrochloric acid, until LC MS/MS analysis indicated the complete conversion to hydrolysed or degraded products [ 26, 28].
However, they are rapidly degraded in solutions of sodium hydroxide.
IMC was found to rapidly and substantially degrade in aqueous solution, when in exposure to active chlorine, displaying a pseudo second-order degradation rate constant of 0.042 ± 0.001 L mol−1 s−1, and a half-life period of 23.8 ± 0.6 s.
The conducted experiments showed that this strain is able to degrade styrene in solutions containing this component at the concentration up to 90 g m−3.The tested microorganisms also showed great resistance to inhibition of their growth by styrene.
The sensors degrade after measurement in solution for a long working time, but they can recover to their initial states in a certain duration after washing, typically ∼24 h.
After thawing and washing with IMDM (containing 2% FBS) to remove DMSO, cells were incubated with DNAse to degrade free DNA in solution that would compromise separation efficiency.
Hydrogels were degraded in pronase solution (Sigma Aldrich, St . Louis MO, 1 mg/mL) to isolate the remaining virus from the gels.
In summary, gentamicin-loaded PTMC discs degrading in lipase solution showed antibiotic release kinetics and biofilm inhibition properties that are comparable to those of non-biodegradable gentamicin-loaded PMMA beads.
