After two hours of incubation, T7cdr2 protein was significantly more degraded than T7cdr2 KEN/D1D2 (Fig. 2C; 40% degradation for wild type compared to 20% degradation for KEN/D1D2; (p<0.05), while cyclinB1 was degraded at the same rate as T7cdr2.
The hydraulic retention time (HRT) was shorter when the HLR was up-regulated; therefore, the interception and the absorption functions of the EF were degraded at the same time.
Plasminogen which is tightly bound to its ligands is probably degraded at the same time as the ligand.
Because assembly of the TTSS is sequential starting with membrane-bound components [19], and that O157 H7 polycistronic TTSS mRNAs are not degraded at the same rate [28], it is possible that transcription levels were determined at a moment of temporal overlap between the two clades.
This equation assumes that αCaMKII degrades at the same rates both in free and bound forms.
The non-phosphorylated wild-type and phosphomimetic mutant Class-2 PEPCs were degraded at the same rate, arguing against a role for Ser phosphorylation in mediating proteolytic BTPC turnover in developing COS.
By this scaling (scaling factors for mRNA based on fit G(t ) function = 1.0 at 0 hr, 0.93 at 1.5 hr, 0.91 at 3 hr, 0.48 at 6 hr, 0.17 at 9 hr, 0.12 at 12 hr), assuming that all mRNA are degraded at the same rate, the absolute mRNA abundance can be estimated across all cells in the population.
Interestingly, elevated levels of antibodies against histone proteins were only associated with reduced ability to degrade NETs at the same time point as the decreased degradation occurred.
With a bone glue, for example, "you want it to degrade roughly at the same rate as the bone regrows," he said.
