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This degradation sequence occurs despite the Fe layer remaining chemically unchanged.
report a photosensitive degron (psd) consisting of the LOV2 domain fused to a protein degradation sequence.
Quantum chemical calculation using the density function theory (DFT) method was combined with experimental results to describe the degradation sequence of TFs.
In addition, with the aid of quantum chemistry calculation and LC/MS analysis, a plausible degradation sequence of DTPA on BDD anode was also proposed.
In addition, a possible degradation sequence involving all the detected intermediates was proposed for the anodic oxidation of BAA on BDD electrode.
Edman degradation sequence and matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) analyses of proteolytic activity have been utilized to aid in further substrate design.
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The complete material balance, and the main degradation sequences are provided within the manuscript.
The first 15 amino acids of the N-terminal sequence of IL-TIL was further confirmed by Edman degradation sequencing to be the same as the sequence deduced from its cDNA.
The putative HMWP2 band from EHOS 03-819 was confirmed by Edman degradation sequencing of the first eight amino acids, which were enough to identify HMWP2.
The scissor indicates the cleavage position predicted by the Edman degradation sequencing.
The bands of interest were cut out of the membrane and analyzed by N-terminal Edman degradation sequencing at the JHMI Synthesis and Sequencing Facility.
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