Shear degradation, concentration effects, viscous fingering, poor column resolution, and in general, low reproducibility are the main difficulties in SEC fractionation.
The particulate degradation concentration is expressed relative to the biomass because the particulate hydrolysis is related to the relative contact area between the non-soluble organic material and the biomass (Metcalf and Eddy 2003).
The baking temperature, rate of saccharose degradation, concentration of reducing sugars, type of sugar (glucose, fructose or others), water activity, the addition of other food additives such as HMF-containing sweeteners, coloring agents, caramelization, storage time and temperature, type of metallic storage and processing container vary widely among different food items.
Although the concentration of Si and Co increased due to bioglass degradation, the concentrations of P and Ca decreased after 3 days, which was attributed to ion precipitation on bioactive glass nanoparticles.
However, in order to avoid fast degradation, tars concentrations have to be below a critical threshold.
For effects of metal ions on the degradation, different concentrations of MgCl2, CuSO4, MnCl2, or ZnCl2 were used.
Digoxin induced both autolysosome formation and degradation at concentrations ranging from 10 μg/ml to 10 ng/ml.
Most of the small hydrophobic ligands could induce protein degradation at concentrations above 10 nM, resulting in cell death.
Hsp90 inhibitors induce client protein degradation at concentrations that mirror their antiproliferative IC50 value, as client protein degradation manifests a direct effect on cell growth.
Only for A-type peptides, with observed ex vivo degradation, lower concentrations were measured, suggesting increased protease activity by the gel.
