Exact(22)
For reversal of the FKBP-Asf1a degradation, cells were incubated with nocodazole in the absence of Shld1 for 18 hours and Shld1 was added for 13 additional hours.
To assess whether the aggregates were removed by 17-AAG-stimulated lysosomal degradation, cells were treated with the lysosomal inhibitor NH4Cl (50 mM) for 24 h either alone or in combination with 17-AAG (50 nM).
To inhibit proteasome-dependent degradation cells were incubated for 16 h in the presence of 5µM Lactacystine and 10 mM MG-132 (Sigma), and protein synthesis was blocked using 50µM of cycloheximide [30].
On the other hand, in mutant strains that are resistant to Sic1 degradation, cells experience lengthened G1 phase in an otherwise wild-type genetic background [20] or G1 phase arrest in more complex situations [8], [21].
For detecting protein degradation, cells were treated with cisplatin, nicotine or both.
For analysis of β-catenin degradation, cells were pre-treated with MG132 for 4 h.
Similar(38)
Additionally, CEL20 induced clients degradation, cell cycle arrest and apoptosis in Panc-1 cells.
Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
The operation of the cell through several thermal cycles is steady and the rate of degradation cell is low.
In conclusion, a three-dimensional ChG/nHaP scaffold was successfully fabricated and characterized in terms of swelling, degradation, cell proliferation, cell attachment, and mineralization characterizations.
The scaffolds were prepared with two different degradation rates in order to study relationships between matrix degradation, cell metabolism and bone tissue formation in vitro.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com