The HPLC system consists of the following accessory instruments: a detector (ELSD, Alltech 3300), quaternary gradient pump (Model 726, Alltech), degasser (Model 591500M Elite degassing system, Alltech), autosampler (Model 570, Alltech), and system controller (Model 726300M, Alltech).
All solutions were degassed with a ThermoVac instrument (MicroCal) before use, and measurements were taken at 25 °C with a MicroCal iTC200 instrument (GE Healthcare).
Samples were diluted to a protein concentration of 5 μM, degassed using a ThermoVac instrument and subsequent measurements were performed on a VP-DSC Capillary Cell MicroCalorimeter (MicroCal, North-hampton, MA).
Brunauer, Emmett and Teller (BET) surface area measurement of the samples was performed using a Beckman Coulter SA 3100 adsorption instrument (Beckman Coulter Inc., Brea, CA, USA) with N2 as adsorbent after degassing the samples at 250°C for 2 h.
Briefly, protein samples were degassed with repeated cycles of purging with argon followed by degassing under vacuum.
For nitrogen sorption measurements, the calcined samples were degassed under vacuum at 300 °C for 3 h and measured on a NOVA 4000e (Quantachrome Instruments, USA) at 77 K in the relative pressure range of p/ p0 = 0.05 0.99.
The samples were not degassed.
Samples were degassed prior to use.
All buffers were degassed before use.
Samples were degassed prior to scanning.
